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Protocol for the pentagastrin test

Authoring team

The pentagastrin test is correctly performed in the following manner:

  • the patient is fasted overnight
  • a nasogastric tube is inserted under fluoroscopy so that the tip lies in the antrum of the stomach
  • basal acid secretion is determined by continuous aspiration of the gastric fluid for one hour
  • pentagastrin is administered (6 mcg/kg i.m.)
  • stimulated acid secretion is determined by continuous aspiration of the gastric fluid for one hour
  • basal and stimulated samples are analysed separately
  • the pH of each is measured
  • each aspirate is titrated against sodium hydroxide to determine the acid production

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