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Mediastinal pleura is the portion of the parietal pleural membrane that lines the mediastinum. It is bounded by and is continuous with the anterior and posterior margins of the costal pleura, the cervical pleura superiorly and the diaphragmatic pleura inferiorly.
At the root of the lung on both sides, the mediastinal parietal pleura passes laterally along the structures of the root to merge with the visceral pleura. This region is the isthmus. Superiorly to the lung root, the mediastinal pleura is a broad, uninterrupted sheet between sternum and vertebrae. Inferior to the lung root, the mediastinal pleura is continuous with the pulmonary ligament.