The polymerase chain reaction is a means of producing a large amount of DNA quickly. Given that the targetted region of DNA is known, a DNA polymerase enzyme, with its nucleotide substrate, is made to produce this region repeatedly by markers to the site at which it starts and finishes - complimentary oligonucleotide primers.
PCR has been used to:
- amplify prenatal samples e.g. chorionic villus for cystic fibrosis
- prepare larger DNA samples for 'fingerprinting' from isolates at crime scenes
- diagnose infectious disease e.g. meningococcal meningitis
- screen for malignancy e.g. for predisposing human papilloma virus in those with cervical carcinoma
Last reviewed 01/2018