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Combined pituitary stimulation test

Last reviewed dd mmm yyyy. Last edited dd mmm yyyy

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The combined pituitary stimulation test is a method for simultaneously assessing hypofunction of anterior pituitary hormones - GH, ACTH, FSH, LH, TSH, and prolactin.

Insulin, GnRH, and TRH are all injected intravenously and the level of pituitary hormones from repeated blood samples taken over a 2 hour period is measured. Blood glucose levels are monitored to ensure the adequacy of the hypoglycaemia.

Hypofunction is confirmed by the lack of response of the appropriate hormone:

  • GH, ACTH - lack of response to insulin
  • FSH, LH - lack of response to GnRH
  • TSH, prolactin - lack of response to TRH

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