The ideal time to check for cardiac troponins is between 6 and 9 hours. Ideally one should also document an elevation in more than one sample. The assays should be performed on serum.
If the onset of symptoms is clear a sample should be taken on admission and between 6-9 hours from onset.
If the onset is indistinct, a sample should be taken on admission, 6 to 9 hours after and optionally measured further at 12 - 24 hours.
High sensitivity cardiac troponins can be taken earlier to determine if an acute myocardial infarction has occurred.
Reference:
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