The diagnosis is usually clinical. If there is doubt then a rise in antibody titres, detection of viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR) techniques or viral culture from saliva, urine or CSF is diagnostic. The excretion of virus persists longer in the urine than in the saliva; it can be cultured from urine in 70% of cases.
Serum
Saliva
Urine
CSF
Reference
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