Post-coital test
This is a test of cervical hostility to spermatozoa.
Procedure:
- test performed at the time of ovulation - determined from rise in patient's basal body temperature, or detection of urinary LH
- intercourse 6 - 12 hours previously
- cervical mucus obtained by syringe, placed on a warm slide, and covered with a coverslip
- with a simple light microscope, assess number and motility of sperm
Interpretation:
- more than 20 motile sperm per high power field - semen penetration is normal i.e. being ejaculated into correct space, sufficient number, no immune problems
- 0-20 motile sperm per high power field - repeat test in a further cycle at time of ovulation
Two negative results should be followed by a cervical mucus test to assess for problems of immunity.
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