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Investigations for microbial keratitis

Last reviewed dd mmm yyyy. Last edited dd mmm yyyy

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  • investigating microbial keratitis
    • corneal scrapings for culture and antibiotic testing is usually employed for bacterial keratitis. This should be done before starting antimicrobial therapy if possible.
    • corneal scrapings for Gram stain, Giemsa stain (2)
    • KOH smear (potassium hydroxide wet mount) has a higher sensitivity than Gram stain, Giemsa stain and Kinyoun's acid-fast stain in diagnosis of infective keratitis caused by fungi, Nocardia and Acanthamoeba (1)
    • PCR


  1. Bharathi MJ et al. Microbiological diagnosis of infective keratitis: comparative evaluation of direct microscopy and culture results. Bharathi MJ Br J Ophthalmol. 2006 Oct;90(10):1271-6.
  2. Update on the Management of Infectious Keratitis - Ophthalmology ( (

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