protocol for 14-C triolein breath test

Last reviewed 01/2018

The protocol is as follows:

  • the patient is fasted overnight
  • collect a basal sample of expired air
  • administer 10 microCi of 14C-triolein in a 60 g fat meal
  • collect samples of expired air each hour for 7 hours
  • measure the 14C in the expired air


  • the 14C activity in expired air is significantly reduced in patients with fat malabsorption